Secondary plant products from cultured hybrid cells.

Secondary Products from Plant Tissue Culture

Cryopreservation of Cultured Plant Cells

Undifferentiated cell cultures of higher plants are important genetic resources used extensively in cytological, physiological, and molecular biological studies. The development of a reliable and cost-effective method for the maintenance of cultured cells is required to minimize the risk of losing cell lines to disease, contamination, and technical errors, as well as the risk of changes in morp...

Selenium elicitation and increase of secondary metabolites production by suspension-cultured Astragalus verus cells

Secondary metabolites with low molecular weight and high economic, pharmaceutical and industrial values play an important role in protecting plants against biotic and abiotic stresses. Astragalus is a medicinal herb, rich in secondary metabolites. Selenium (Se) is an essential element for humans, animals, many bacteria, and a beneficial element for plants. The plants response to selenium is dep...

Conditioned Medium from Cultured Colorectal Cancer Cells Affects Peripheral Blood Mononuclear Cells Inflammatory Phenotype in Vitro

Background: Colorectal cancer (CRC) is the third most common cancer worldwide. Studies have indicated that immune cells and soluble factors play a key role in maintaining the balance between tumor-promoting inflammation and anti-tumor immunity. It has been shown that secreted cytokines from CRC cell lines could affect peripheral blood mononuclear cells (PBMCs), monocytes, and macrophages phenot...

CFU-GM Like Colonies Derived from Embryonic Stem Cells Cultured on the Bone Marrow Stromal Cells

The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...